Characterization of the main steps in first shell formation in Mytilus galloprovincialis: possible role of tyrosinase.

ABSTRACT: Bivalve biomineralization is a highly complex and organized process, involving several molecular components identified in adults and larval stages. However, information is still scarce on the ontogeny of the organic matrix before calcification occurs. In this work, first shell formation was investigated in the mussel Mytilus galloprovincialis. The time course of organic matrix and CaCO₃ deposition were followed at close times post fertilization (24, 26, 29, 32, 48 h) by calcofluor and calcein staining, respectively. Both components showed an exponential trend in growth, with a delay between organic matrix and CaCO₃ deposition. mRNA levels of genes involved in matrix deposition (chitin synthase; tyrosinase- TYR) and calcification (carbonic anhydrase; extrapallia

SUBMITTER: Miglioli A 

PROVIDER: S-EPMC6939270 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature