Exploring Relationships between the Density of Charged Tracts within Disordered Regions and Phase Separation.

ABSTRACT: Biomolecular condensates form through a process termed phase separation and play diverse roles throughout the cell. Proteins that undergo phase separation often have disordered regions that can engage in weak, multivalent interactions; however, our understanding of the sequence grammar that defines which proteins phase separate is far from complete. Here, we show that proteins that display a high density of charged tracts within intrinsically disordered regions are likely to be constituents of electrostatically organized biomolecular condensates. We scored the human proteome using an algorithm termed ABTdensity that quantifies the density of charged tracts and observed that proteins with more charged tracts are enriched in particular Gene Ontology annotations and, based upon analysis of in

SUBMITTER: Somjee R 

PROVIDER: S-EPMC6939312 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature