Evolutionary superscaffolding and chromosome anchoring to improve Anopheles genome assemblies.

ABSTRACT:

Background

New sequencing technologies have lowered financial barriers to whole genome sequencing, but resulting assemblies are often fragmented and far from 'finished'. Updating multi-scaffold drafts to chromosome-level status can be achieved through experimental mapping or re-sequencing efforts. Avoiding the costs associated with such approaches, comparative genomic analysis of gene order conservation (synteny) to predict scaffold neighbours (adjacencies) offers a potentially useful complementary method for improving draft assemblies.

Results

We evaluated and employed 3 gene synteny-based methods applied to 21 Anopheles mosquito assemblies to produce consensus sets of scaffold adjacencies. For subsets of the assemblies, we integrated these with additional supporting data t

SUBMITTER: Waterhouse RM 

PROVIDER: S-EPMC6939337 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature