Alcohol withdrawal upregulates mRNA encoding for Ca_V2.1-α1 subunit in the rat inferior colliculus.

ABSTRACT: We previously reported increased current density through P-type voltage-gated Ca²⁺ channels in inferior colliculus (IC) neurons during alcohol withdrawal. However, the molecular correlate of this increased P-type channel current is currently unknown. Here, we probe changes in mRNA and protein expression of the pore-forming Ca_V2.1-α1 (P/Q-type) subunits in IC neurons during the course of alcohol withdrawal-induced seizures (AWSs). Rats received three daily doses of ethanol or the vehicle every 8 h for 4 consecutive days. The IC was dissected at various time intervals following alcohol withdrawal, and the mRNA and protein levels of the Ca_V2.1-α1 subunits were measured. In separate experiments, rats were tested for acoustically evoked seizure susceptibility 3,

SUBMITTER: Newton J 

PROVIDER: S-EPMC6939454 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature