Multilayer intraclonal heterogeneity in chronic myelomonocytic leukemia.

ABSTRACT: The functional diversity of cells that compose myeloid malignancies, i.e., the respective roles of genetic and epigenetic heterogeneity in this diversity, remains poorly understood. This question is addressed in chronic myelomonocytic leukemia, a myeloid neoplasm in which clinical diversity contrasts with limited genetic heterogeneity. To generate induced pluripotent stem cell clones, we reprogrammed CD34⁺ cells collected from a patient with a chronic myelomonocytic leukemia in which whole exome sequencing of peripheral blood monocyte DNA had identified 12 gene mutations, including a mutation in KDM6A and two heterozygous mutations in TET2 in the founding clone and a secondary KRAS(G12D) mutation. CD34⁺ cells from an age-matched healthy donor were

SUBMITTER: Beke A 

PROVIDER: S-EPMC6939510 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature