Temporal Impact of Substrate Anisotropy on Differentiating Cardiomyocyte Alignment and Functionality.

ABSTRACT: Anisotropic biomaterials can affect cell function by driving cell alignment, which is critical for cardiac engineered tissues. Recent work, however, has shown that pluripotent stem cell-derived cardiomyocytes may self-align over long periods of time. To determine how the degree of biomaterial substrate anisotropy impacts differentiating cardiomyocyte structure and function, we differentiated mouse embryonic stem cells to cardiomyocytes on nonaligned, semialigned, and aligned fibrous substrates and evaluated cell alignment, contractile displacement, and calcium transient synchronicity over time. Although cardiomyocyte gene expression was not affected by fiber alignment, we observed gradient- and threshold-based differences in cardiomyocyte alignment and function. Cardiomyocyte alignment inc

SUBMITTER: Allen ACB 

PROVIDER: S-EPMC6939589 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature