Shared reference materials harmonize lipidomics across MS-based detection platforms and laboratories.

ABSTRACT: Quantitative MS of human plasma lipids is a promising technology for translation into clinical applications. Current MS-based lipidomic methods rely on either direct infusion (DI) or chromatographic lipid separation methods (including reversed phase and hydrophilic interaction LC). However, the use of lipid markers in laboratory medicine is limited by the lack of reference values, largely because of considerable differences in the concentrations measured by different laboratories worldwide. These inconsistencies can be explained by the use of different sample preparation protocols, method-specific calibration procedures, and other experimental and data-reporting parameters, even when using identical starting materials. Here, we systematically investigated the roles of some of these variabl

SUBMITTER: Triebl A 

PROVIDER: S-EPMC6939597 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature