Evaluation of freely available software tools for untargeted quantification of ¹³C isotopic enrichment in cellular metabolome from HR-LC/MS data.

ABSTRACT: ¹³C Metabolic Flux Analysis (¹³C-MFA) involves the quantification of isotopic enrichment in cellular metabolites and fitting the resultant data to the metabolic network model of the organism. Coverage and resolution of the resultant flux map depends on the total number of metabolites and fragments in which ¹³C enrichment can be quantified accurately. Experimental techniques for tracking ¹³C enrichment are evolving rapidly and large volumes of data are now routinely generated through the use of Liquid Chromatography coupled with High-Resolution Mass Spectrometry (HR-LC/MS). Therefore, the current manuscript is focused on the challenges in high-throughput analyses of such large datasets. Current ¹³C-MFA studies often have to rely on the

SUBMITTER: Dange MC 

PROVIDER: S-EPMC6940703 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature