Membrane Transporters in Citrus clementina Fruit Juice-Derived Nanovesicles.

ABSTRACT: The cellular vesicle is a fluid-filled structure separated from the surrounding environment by a biological membrane. Here, we isolated nanovesicles (NVs) from the juice of clementines using a discontinuous density gradient ultracentrifugation method. To gain information about the protein content of vesicles, mass spectrometry-based organelle proteomics and bioinformatics were applied to the exosome-like vesicle fraction isolated in the 1 mol/L sucrose/D₂O cushion. Analysis of 1018 identified proteins revealed a highly complex mixture of different intra, extracellular and artificially-formed vesicle populations. In particular, clathrin-coated vesicles were significantly expressed in this sample. Membrane transporters are significantly represented in clementines nanovesicles. We

SUBMITTER: Stanly C 

PROVIDER: S-EPMC6941005 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature