Sphingosine-1-Phosphate Enhances α₁-Adrenergic Vasoconstriction via S1P2-G_12/13-ROCK Mediated Signaling.

ABSTRACT: Sphingosine-1-phosphate (S1P) has been implicated recently in the physiology and pathology of the cardiovascular system including regulation of vascular tone. Pilot experiments showed that the vasoconstrictor effect of S1P was enhanced markedly in the presence of phenylephrine (PE). Based on this observation, we hypothesized that S1P might modulate α₁-adrenergic vasoactivity. In murine aortas, a 20-minute exposure to S1P but not to its vehicle increased the E_max and decreased the EC₅₀ of PE-induced contractions indicating a hyperreactivity to α₁-adrenergic stimulation. The potentiating effect of S1P disappeared in S1P2 but not in S1P3 receptor-deficient vessels. In addition, smooth muscle specific conditional deletion of G_12/13 protein

SUBMITTER: Panta CR 

PROVIDER: S-EPMC6941080 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature