Evaporation-Driven Micromixing in Sessile Droplets for Miniaturized Absorbance-Based Colorimetry.

ABSTRACT: We demonstrate the use of an evaporating, sessile droplet on a nonwetting substrate as a miniature micromixing device to conduct sample-dye reactions for absorbance-based colorimetry. The nonwetting substrate supports buoyancy-induced mixing inside the droplet for rapid completion of the measurement. The Bradford assay is used as a proof of concept, where a protein-containing sample is reacted with a reagent dye to measure the protein concentration. Viability of absorbance measurement through the droplet is first established using droplets in which the reactants are mixed prior to their deposition onto the substrate. In a second set of experiments involving in situ mixing, the reagent is directly added to a sessile droplet of the protein-containing sample, allowing the reactants to mix whi

SUBMITTER: Chandramohan A 

PROVIDER: S-EPMC6941180 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature