Reconstruction and analysis of a carbon-core metabolic network for Dunaliella salina.

ABSTRACT:

Background

The green microalga Dunaliella salina accumulates a high proportion of β-carotene during abiotic stress conditions. To better understand the intracellular flux distribution leading to carotenoid accumulation, this work aimed at reconstructing a carbon core metabolic network for D. salina CCAP 19/18 based on the recently published nuclear genome and its validation with experimental observations and literature data.

Results

The reconstruction resulted in a network model with 221 reactions and 212 metabolites within three compartments: cytosol, chloroplast and mitochondrion. The network was implemented in the MATLAB toolbox CellNetAnalyzer and checked for feasibility. Furthermore, a flux balance analysis was carried out for different light and nutrient uptake rates.

SUBMITTER: Fachet M 

PROVIDER: S-EPMC6941287 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature