Bile and urine peptide marker profiles: access keys to molecular pathways and biological processes in cholangiocarcinoma.

ABSTRACT:

Background

Detection of cholangiocarcinoma (CCA) remains a diagnostic challenge. We established diagnostic peptide biomarkers in bile and urine based on capillary electrophoresis coupled to mass spectrometry (CE-MS) to detect both local and systemic changes during CCA progression. In a prospective cohort study we recently demonstrated that combined bile and urine proteome analysis could further improve diagnostic accuracy of CCA diagnosis in patients with unknown biliary strictures. As a continuation of these investigations, the aim of the present study was to investigate the pathophysiological mechanisms behind the molecular determinants reflected by bile and urine peptide biomarkers.

Methods

Protease mapping and gene ontology cluster analysis were performed for the previou

SUBMITTER: Voigtlander T 

PROVIDER: S-EPMC6941325 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature