Adenylyl Cyclase 6 Expression Is Essential for Cholera Toxin-Induced Diarrhea.

ABSTRACT:

Background

Cholera toxin (CT)-induced diarrhea is mediated by cyclic adenosine monophosphate (cAMP)-mediated active Cl- secretion via the cystic fibrosis transmembrane conductance regulator (CFTR). Although the constitutive activation of adenylyl cyclase (AC) in response to CT is due to adenosine diphosphate ribosylation of the small G protein α-subunit activating CFTR with consequent secretory diarrhea, the AC isoform(s) involved remain unknown.

Methods

We generated intestinal epithelial cell-specific adenylyl cyclase 6 (AC6) knockout mice to study its role in CT-induced diarrhea.

Results

AC6 messenger RNA levels were the highest of all 9 membrane-bound AC isoforms in mouse intestinal epithelial cells. Intestinal epithelial-specific AC6 knockout mice (AC6loxloxVillin

SUBMITTER: Fenton RA 

PROVIDER: S-EPMC6941499 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature