Flexible Cobamide Metabolism in Clostridioides (Clostridium) difficile 630 Δerm.

ABSTRACT: Clostridioides (Clostridium) difficile is an opportunistic pathogen known for its ability to colonize the human gut under conditions of dysbiosis. Several aspects of its carbon and amino acid metabolism have been investigated, but its cobamide (vitamin B₁₂ and related cofactors) metabolism remains largely unexplored. C. difficile has seven predicted cobamide-dependent pathways encoded in its genome in addition to a nearly complete cobamide biosynthesis pathway and a cobamide uptake system. To address the importance of cobamides to C. difficile, we studied C. difficile 630 Δerm and mutant derivatives under cobamide-dependent conditions in vitro Our results show that C. difficile can use a surprisingly diverse array of

SUBMITTER: Shelton AN 

PROVIDER: S-EPMC6941526 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature