Characterization of rapid neutrophil extracellular trap formation and its cooperation with phagocytosis in human neutrophils.

ABSTRACT: Neutrophils, as the first cellular line of innate host defense, employ phagocytosis and formation of neutrophil extracellular traps (NETs) to combat infections. Classical NET formation induced by phorbol myristate acetate requires several hours to complete. However, recent studies demonstrated rapid NET formation in neutrophils upon stimulation by platelets, Staphylococcus aureus or fungal products. Here we describe that antibody- or complement-induced phagocytosis triggers rapid NET formation. In contrast to classical NETosis, chemical inhibition of NADPH oxidase as well as using NADPH oxidase-deficient patient neutrophils did not affect rapid NET formation. Although phagocytosis and rapid NET formation may not be the prerequisite of each other, cooperation of phagocytosis and rapid NET f

SUBMITTER: Saffarzadeh M 

PROVIDER: S-EPMC6941580 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature