Plasma proteome profiling of freshwater and seawater life stages of rainbow trout (Oncorhynchus mykiss).

ABSTRACT: The sea-run phenotype of rainbow trout (Oncorhynchus mykiss), like other anadromous salmonids, present a juvenile stage fully adapted to life in freshwater known as parr. Development in freshwater is followed by the smolt stage, where preadaptations needed for seawater life are developed making fish ready to migrate to the ocean, after which event they become post-smolts. While these three life stages have been studied using a variety of approaches, proteomics has never been used for such purpose. The present study characterised the blood plasma proteome of parr, smolt and post-smolt rainbow trout using a gel electrophoresis liquid chromatography tandem mass spectrometry approach alone or in combination with low-abundant protein enrichment technology (combinatorial peptide ligand library).

SUBMITTER: Morro B 

PROVIDER: S-EPMC6941806 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature