Micturition video thermography in awake, behaving mice.

ABSTRACT:

Background

Our understanding of the neural circuits controlling micturition and continence is constrained by a paucity of techniques for measuring voiding in awake, behaving mice.

New method

To facilitate progress in this area, we developed a new, non-invasive assay, micturition video thermography (MVT), using a down-facing thermal camera above mice on a filter paper floor.

Results

Most C57B6/J mice void infrequently, with a stereotyped behavioral sequence, and usually in a corner. The timing of each void is indicated by the warm thermal contrast of freshly voided urine. Over the following 10-15 min, urine cools to ∼3 °C below the ambient temperature and spreads radially in the filter paper. By measuring the area of cool contrast comprising this "thermal void spot," w

SUBMITTER: Verstegen AM 

PROVIDER: S-EPMC6942227 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature