Prokaryotic ribosomal RNA stimulates zebrafish embryonic innate immune system.

ABSTRACT: OBJECTIVES:Cell-culture studies reported that prokaryotic RNA molecules among the various microbe-associated molecular patterns (MAMPs) were uniquely present in live bacteria and were categorized as viability-associated MAMPs. They also reported that specific nucleotide modifications are instrumental in the discrimination between self and nonself RNAs. The aim of this study was to characterize the in vivo immune induction potential of prokaryotic and eukaryotic ribosomal RNAs (rRNAs) using zebrafish embryos as novel whole animal model system. Additionally, we aimed to test the possible role of rRNA modifications in immune recognition. RESULTS:We used three immune markers to evaluate the induction potential of prokaryotic rRNA derived from Escherichia coli and eukaryotic rRNAs from chicken

SUBMITTER: Basu A 

PROVIDER: S-EPMC6942260 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature