Age-dependent impairment of adipose-derived stem cells isolated from horses.

ABSTRACT:

Background

Progressive loss of cell functionality caused by an age-related impairment in cell metabolism concerns not only mature specialized cells but also its progenitors, which significantly reduces their regenerative potential. Adipose-derived stem cells (ASCs) are most commonly used in veterinary medicine as an alternative treatment option in ligaments and cartilage injuries, especially in case of high-value sport horses. Therefore, the main aim of this study was to identify the molecular alternations in ASCs derived from three age-matched horse groups: young (< 5), middle-aged (5-15), and old (> 15 years old).

Methods

ASCs were isolated from three age-matched horse groups using an enzymatic method. Molecular changes were assessed using qRT-PCR, ELISA and western blot m

SUBMITTER: Alicka M 

PROVIDER: S-EPMC6942290 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature