Role of offset and gradient architectures of 3-D melt electrowritten scaffold on differentiation and mineralization of osteoblasts.

ABSTRACT:

Background

Cell-scaffold based therapies have the potential to offer an efficient osseous regenerative treatment and PCL has been commonly used as a scaffold, however its effectiveness is limited by poor cellular retention properties. This may be improved through a porous scaffold structure with efficient pore arrangement to increase cell entrapment. To facilitate this, melt electrowriting (MEW) has been developed as a technique able to fabricate cell-supporting scaffolds with precise micro pore sizes via predictable fibre deposition. The effect of the scaffold's architecture on cellular gene expression however has not been fully elucidated.

Methods

The design and fabrication of three different uniform pore structures (250, 500 and 750 μm), as well as two offset scaffolds wi

SUBMITTER: Abbasi N 

PROVIDER: S-EPMC6942301 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature