Cellular therapy of corneal epithelial defect by adipose mesenchymal stem cell-derived epithelial progenitors.

ABSTRACT:

Background

Persistent epithelial defects (PED), associated with limbal stem cell deficiency (LSCD), require ocular surface reconstruction with a stable corneal epithelium (CE). This study investigated CE reformation using human adipose mesenchymal stem cells (ADSC), which derived epithelial progenitors via mesenchymal-epithelial transition (MET).

Methods

STEMPRO human ADSC were cultured with specific inhibitors antagonizing glycogen synthase kinase-3 and transforming growth factor-β signaling, followed by culture under a defined progenitor cell targeted-epithelial differentiation condition to generate epithelial-like cells (MET-Epi), which were characterized for cell viability, mesenchymal, and epithelial phenotypes using immunofluorescence and flow cytometry. Tissue-enginee

SUBMITTER: Bandeira F 

PROVIDER: S-EPMC6942321 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature