Influence of mesenchymal stem cell-derived extracellular vesicles in vitro and their role in ageing.

ABSTRACT:

Introduction

This study assessed whether mesenchymal stem cell (MSC)-derived extracellular vesicles influenced ageing and pluripotency markers in cell cultures where they are added.

Methods

MSC-derived extracellular vesicles from old and young rat bone marrows were isolated by ultracentrifugation and were characterised by western blotting, nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM). They were added to young and old MSC cultures. Real-time quantitative reverse transcription polymerase chain reactions and western blot analysis were performed to check the markers of ageing (vinculin and lamin A), pluripotency markers (Nanog and Oct4) and components of the mTOR signalling pathway (Rictor, Raptor, AKT and mTOR) in these cell populations. Subse

SUBMITTER: Fafian-Labora J 

PROVIDER: S-EPMC6942375 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature