Preparation of the inactivated Newcastle disease vaccine by plasma activated water and evaluation of its protection efficacy.

ABSTRACT: Vaccination has been regarded as the most effective way to reduce death and morbidity caused by infectious diseases in the livestock industry. In this study, plasma activated water (PAW) was introduced to prepare the inactivated Newcastle disease vaccine. Humoral immune response was tested by hemagglutination inhibition (HI) assay and enzyme-linked immunosorbent assay (ELISA). In addition, cell-mediated immune response was evaluated by lymphocyte proliferation assay and flow cytometry. The results demonstrated that the vaccine prepared by PAW at appropriate volume ratio could induce similar antibody titers in specific pathogen-free (SPF) chickens compared with the formaldehyde-inactivated vaccine. The challenge experiment further confirmed that the vaccine prepared by PAW conferred solid p

SUBMITTER: Hongzhuan Z 

PROVIDER: S-EPMC6942578 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature