Induction of immunoproteasomes in porcine kidney (PK)-15 cells by interferon-? and tumor necrosis factor-?.

ABSTRACT: Immunoproteasome (i-proteasome) has both immune and non-immune functions and plays important roles in controlling infections and combating illnesses. Our previous studies suggest that interferon (IFN)-? induces the expression of three immune-specific catalytic subunits of the 20S proteasome that can replace their constitutive homologues to form the i-proteasome in immune cells, such as porcine alveolar macrophages (AMs) in vitro. However, i-proteasome levels and their modulation in non-immune cells such as the epithelial cells in pigs remain unknown. Here, we investigated the expression of i-proteasomes in non-immune cells (porcine kidney (PK)-15 cells) to determine i-proteasome modulation upon stimulation of PK-15 cells with IFN-? and tumor necrosis factor (TNF)-? in vitro. The expression of i-proteasome subunits in PK-15 cells were regulated by IFN-? and TNF-?. Remarkably, we found that the combination treatment of IFN-? and TNF-? increased the expression of i-proteasome subunits LMP2, LMP7, and MECL-1 in PK-15 cells at transcriptional levels, but may decrease their expression at translational level, compared to their expression levels induced by individual cytokine treatments. These results provide critical insight into i-proteasome modulation in porcine non-immune cells, contribute further to our understanding of i-proteasome function in tissue pathogenesis and the development of antiviral adaptive immune responses against intracellular infections.

SUBMITTER: Liu Q 

PROVIDER: S-EPMC6943335 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature