Increased HLA-DR expression and cortical demyelination in MS links with HLA-DR15.

ABSTRACT: To investigate molecular changes in multiple sclerosis (MS) normal-appearing cortical gray matter (NAGM). We performed a whole-genome gene expression microarray analysis of human brain autopsy tissues from 64 MS NAGM samples and 42 control gray matter samples. We further examined our cases by HLA genotyping and performed immunohistochemical and immunofluorescent analysis of all human brain tissues. HLA-DRB1 is the transcript with highest expression in MS NAGM with a bimodal distribution among the examined cases. Genotyping revealed that every case with the MS-associated HLA-DR15 haplotype also shows high HLA-DRB1 expression and also of the tightly linked HLA-DRB5 allele. Quantitative immunohistochemical analysis confirmed the higher expression of HLA-DRB1 in HLA-DRB1*15:01 cases at the pro

SUBMITTER: Enz LS 

PROVIDER: S-EPMC6943368 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature