An exploration of smokeless tobacco product nucleic acids: a combined metagenome and metatranscriptome analysis.

ABSTRACT: Smokeless tobacco (ST) products are used worldwide and are a major public health concern. In addition to harmful chemicals found in these products, microbes found in ST products are believed to be responsible for generating harmful tobacco-specific nitrosamines (TSNAs), the most abundant carcinogens in ST. These microbes also contribute endotoxins and other pro-inflammatory components. A greater understanding of the microbial constituents in these products is sought in order to potentially link select design aspects or manufacturing processes to avoidable increases in harmful constituents. Previous studies looked primarily at bacterial constituents and had not differentiated between viable vs nonviable organisms, so in this study, we sought to use a dual metatranscriptomic and metagenomic

SUBMITTER: Tyx RE 

PROVIDER: S-EPMC6943401 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature