Functional analysis of the heterotrimeric NF-Y transcription factor complex in cassava disease resistance.

ABSTRACT:

Background and aims

The nuclear factor Y (NF-Y) transcription factor complex is important in plant growth, development and stress response. Information regarding this transcription factor complex is limited in cassava (Manihot esculenta). In this study, 15 MeNF-YAs, 21 MeNF-YBs and 15 MeNF-YCs were comprehensively characterized during plant defence.

Methods

Gene expression in MeNF-Ys was examined during interaction with the bacterial pathogen Xanthomonas axonopodis pv. manihotis (Xam). The yeast two-hybrid system was employed to investigate protein-protein interactions in the heterotrimeric NF-Y transcription factor complex. The in vivo roles of MeNF-Ys were revealed by virus-induced gene silencing (VIGS) in cassava.

Key results

The regulation of MeNF-Ys in response t

SUBMITTER: He X 

PROVIDER: S-EPMC6943695 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature