Reconstruction of Cell-type-Specific Interactomes at Single-Cell Resolution.

ABSTRACT: The human interactome is instrumental in the systems-level study of the cell and the contextualization of disease-associated gene perturbations. However, reference organismal interactomes do not capture the cell-type-specific context in which proteins and modules preferentially act. Here, we introduce SCINET, a computational framework that reconstructs an ensemble of cell-type-specific interactomes by integrating a global, context-independent reference interactome with a single-cell gene-expression profile. SCINET addresses technical challenges of single-cell data by robustly imputing, transforming, and normalizing the initially noisy and sparse expression of data. Inferred cell-level gene interaction probabilities and group-level interaction strengths define cell-type-specific interactome

SUBMITTER: Mohammadi S 

PROVIDER: S-EPMC6943823 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature