Genetic variations on 31 and 450 residues of influenza A nucleoprotein affect viral replication and translation.

ABSTRACT:

Background

Influenza A viruses cause epidemics/severe pandemics that pose a great global health threat. Among eight viral RNA segments, the multiple functions of nucleoprotein (NP) play important roles in viral replication and transcription.

Methods

To understand how NP contributes to the virus evolution, we analyzed the NP gene of H3N2 viruses in Taiwan and 14,220 NP sequences collected from Influenza Research Database. The identified genetic variations were further analyzed by mini-genome assay, virus growth assay, viral RNA and protein expression as well as ferret model to analyze their impacts on viral replication properties.

Results

The NP genetic analysis by Taiwan and global sequences showed similar evolution pattern that the NP backbones changed through time a

SUBMITTER: Hung SJ 

PROVIDER: S-EPMC6943894 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature