Optimization of a Collagen-Targeted PET Probe for Molecular Imaging of Pulmonary Fibrosis.

ABSTRACT: There is a large unmet need for a simple, accurate, noninvasive, quantitative, and high-resolution imaging modality to detect lung fibrosis at early stage and to monitor disease progression. Overexpression of collagen is a hallmark of organ fibrosis. Here, we describe the optimization of a collagen-targeted PET probe for staging pulmonary fibrosis. Methods: Six peptides were synthesized, conjugated to a copper chelator, and radiolabeled with ⁶⁴Cu. The collagen affinity of each probe was measured in a plate-based assay. The pharmacokinetics and metabolic stability of the probes were studied in healthy rats. The capacity of these probes to detect and stage pulmonary fibrosis in vivo was assessed in a mouse model of bleomycin-induced fibrosis using PET imaging. Results:</

SUBMITTER: Desogere P 

PROVIDER: S-EPMC6944164 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature