Prediction of Conformation Specific Thermostabilizing Mutations for Class A G Protein-Coupled Receptors.

ABSTRACT: G protein-coupled receptors (GPCRs) are highly flexible and prone to denaturation during protein extraction in detergents and purification. This poses a huge challenge to purify a conformationally homogeneous solution of GPCRs. Thermostabilizing mutations have been used widely to purify and obtain crystal structures of several GPCRs. However, identifying thermostabilizing mutations for GPCRs remains a tedious and expensive task as they are not transferable even among closely related GPCRs. Additionally, the mutations stabilizing one conformational state of a GPCR do not always stabilize other conformational state(s) of the same GPCR. Previously we developed a computational method, LiticonDesign, for rapid prediction of thermostabilizing mutations for a specific GPCR conformation. In this s

SUBMITTER: Jana S 

PROVIDER: S-EPMC6944194 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature