Macrophages display proinflammatory phenotypes in the eutopic endometrium of women with endometriosis with relevance to an infectious etiology of the disease.

ABSTRACT:

Objective

To phenotype transcriptomically M1 macrophages (Mϕ1) and M2 macrophages (Mϕ2) in the endometrium of women with endometriosis.

Design

Prospective experimental study.

Setting

University research laboratory.

Patient(s)

Six women with endometriosis and five controls without disease, in the secretory phase of the menstrual cycle.

Intervention(s)

Mϕ1, Mϕ2, uterine natural killer, and T regulatory cells were isolated from human endometrium using a uniquely designed cell-specific fluorescence activating cell sorting panel. Transcriptome profiles were assessed by RNA high sequencing, bioinformatics, and biological pathway analyses.

Main outcomes measure(s)

Differential gene expression between Mϕ1 and Mϕ2 in women with and without endometriosis an

SUBMITTER: Vallve-Juanico J 

PROVIDER: S-EPMC6944306 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature