Prostate tumor growth is impaired by CtBP1 depletion in high-fat diet-fed mice.

ABSTRACT:

Purpose

Clinical and epidemiologic data suggest that obesity is associated with more aggressive forms of prostate cancer, poor prognosis, and increased mortality. C-terminal-binding protein 1 (CtBP1) is a transcription repressor of tumor suppressor genes and is activated by NADH binding. High calorie intake decreases intracellular NAD(+)/NADH ratio. The aim of this work was to assess the effect of high-fat diet (HFD) and CtBP1 expression modulation over prostate xenograft growth.

Experimental design

We developed a metabolic syndrome-like disease in vivo model by feeding male nude mice with HFD during 16 weeks. Control diet (CD)-fed animals were maintained at the same conditions. Mice were inoculated with PC3 cells stable transfected with shCtBP1 or control plasmids. Genome-w

SUBMITTER: Moiola CP 

PROVIDER: S-EPMC6944435 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature