Emissive Synthetic Cofactors: A Highly Responsive NAD⁺ Analogue Reveals Biomolecular Recognition Features.

ABSTRACT: Apart from its vital function as a redox cofactor, nicotinamide adenine dinucleotide (NAD⁺ ) has emerged as a crucial substrate for NAD⁺ -consuming enzymes, including poly(ADP-ribosyl)transferase 1 (PARP1) and CD38/CD157. Their association with severe diseases, such as cancer, Alzheimer's disease, and depressions, necessitates the development of new analytical tools based on traceable NAD⁺ surrogates. Here, the synthesis, photophysics and biochemical utilization of an emissive, thieno[3,4-d]pyrimidine-based NAD⁺ surrogate, termed N^th AD⁺ , are described. Its preparation was accomplished by enzymatic conversion of synthetic ^th ATP by nicotinamide mononucleotide adenylyltransferase 1 (NMNAT1). The new NAD⁺

SUBMITTER: Feldmann J 

PROVIDER: S-EPMC6944523 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature