Live-cell monitoring of protein localization to membrane rafts using protein-fragment complementation.

ABSTRACT: The plasma membrane consists of a variety of discrete domains differing from the surrounding membrane in composition and properties. Selective partitioning of protein to these microdomains is essential for membrane functioning and integrity. Studying the nanoscale size and dynamic nature of the membrane microdomains requires advanced imaging approaches with a high spatiotemporal resolution and, consequently, expensive and specialized equipment, unavailable for most researchers and unsuited for large-scale studies. Thus, understanding of protein partitioning to the membrane microdomains in health and disease is still hampered by the lack of inexpensive live-cell approaches with an appropriate spatial resolution. Here, we have developed a novel approach based on Gaussia princeps luciferase p

SUBMITTER: Merezhko M 

PROVIDER: S-EPMC6944658 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature