A Genetic Screen To Identify Genes Influencing the Secondary Redox Couple NADPH/NADP⁺ in the Yeast Saccharomyces cerevisiae.

ABSTRACT: NADPH is an important cofactor in the cell. In addition to its role in the biosynthesis of critical metabolites, it plays crucial roles in the regeneration of the reduced forms of glutathione, thioredoxins and peroxiredoxins. The enzymes and pathways that regulate NADPH are thus extremely important to understand, and yet are only partially understood. We have been interested in understanding how NADPH fluxes are altered in the cell. We describe here both an assay and a genetic screen that allows one to discern changes in NADPH levels. The screen exploits the secondary redox property of NADPH. At low levels of glutathione we show that the redox contributions of NADPH become critical for growth, and we have used this to develop a genetic screen for genes affecting NADPH homeostasis. The scre

SUBMITTER: Yadav S 

PROVIDER: S-EPMC6945034 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature