Uncoupling protein 1 inhibits mitochondrial reactive oxygen species generation and alleviates acute kidney injury.

ABSTRACT:

Background

Uncoupling protein 1 (UCP1) is predominantly found in brown adipose tissue mitochondria, and mediates energy dissipation to generate heat rather than ATP via functional mitochondrial uncoupling. However, little is known about its expression and function in kidney.

Methods

We carried out a mRNA microarray analysis in mice kidneys with ischemia reperfusion (IR) injury. The most dramatically downregulated gene UCP1 after IR was identified, and its role in generation of mitochondrial reactive oxygen species (ROS) and oxidative stress injury was assessed both in vitro and in vivo. Genetic deletion of UCP1 was used to investigate the effects of UCP1 on ischemia or cisplatin-indued acute kidney injury (AKI) in mice.

Findings

UCP1 was located in renal tubular epith

SUBMITTER: Jia P 

PROVIDER: S-EPMC6945195 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature