Structural basis of polyethylene glycol recognition by antibody.

ABSTRACT:

Background

Polyethylene glycol (PEG) is widely used in industry and medicine. Anti-PEG antibodies have been developed for characterizing PEGylated drugs and other applications. However, the underlying mechanism for specific PEG binding has not been elucidated.

Methods

The Fab of two cognate anti-PEG antibodies 3.3 and 2B5 were each crystallized in complex with PEG, and their structures were determined by X-ray diffraction. The PEG-Fab interactions in these two crystals were analyzed and compared with those in a PEG-containing crystal of an unrelated anti-hemagglutinin 32D6-Fab. The PEG-binding stoichiometry was examined by using analytical ultracentrifuge (AUC).

Results

A common PEG-binding mode to 3.3 and 2B5 is seen with an S-shaped core PEG fragment bound to two dy

SUBMITTER: Lee CC 

PROVIDER: S-EPMC6945545 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature