Patterns of spon1b:GFP expression during early zebrafish brain development.

ABSTRACT: OBJECTIVE:F-spondin is part of a group of evolutionarily conserved extracellular matrix proteins in vertebrates. It is highly expressed in the embryonic floor plate, and it can bind to the ECM and promote neuronal outgrowth. A characterization of F-spondin expression patterns in the adult zebrafish brain was previously reported by our group. However, given its importance during development, we aimed to obtain a detailed description of green fluorescent protein (GFP) expression driven by the spon1b promotor, in the developing zebrafish brain of the transgenic Tg(spon1b:GFP) line, using light sheet fluorescence microscopy (LSFM). RESULTS:Images obtained in live embryos from 22 to 96 h post fertilization confirmed our earlier reports on the presence of spon1b:GFP expressing cells in the telen

SUBMITTER: Agudelo-Duenas N 

PROVIDER: S-EPMC6945593 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature