Genetic disruption of slc4a10 alters the capacity for cellular metabolism and vectorial ion transport in the choroid plexus epithelium.

ABSTRACT:

Background

Genetic disruption of slc4a10, which encodes the sodium-dependent chloride/bicarbonate exchanger Ncbe, leads to a major decrease in Na⁺-dependent HCO₃^- import into choroid plexus epithelial cells in mice and to a marked reduction in brain intraventricular fluid volume. This suggests that Ncbe functionally is a key element in vectorial Na⁺ transport and thereby for cerebrospinal fluid secretion in the choroid plexus. However, slc4a10 disruption results in severe changes in expression of Na⁺,K⁺-ATPase complexes and other major transport proteins, indicating that profound cellular changes accompany the genetic manipulation.

Methods

A tandem mass tag labeling strategy was chosen for quantitative mass sp

SUBMITTER: Christensen IB 

PROVIDER: S-EPMC6945596 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature