Clustered CTCF binding is an evolutionary mechanism to maintain topologically associating domains.

ABSTRACT:

Background

CTCF binding contributes to the establishment of a higher-order genome structure by demarcating the boundaries of large-scale topologically associating domains (TADs). However, despite the importance and conservation of TADs, the role of CTCF binding in their evolution and stability remains elusive.

Results

We carry out an experimental and computational study that exploits the natural genetic variation across five closely related species to assess how CTCF binding patterns stably fixed by evolution in each species contribute to the establishment and evolutionary dynamics of TAD boundaries. We perform CTCF ChIP-seq in multiple mouse species to create genome-wide binding profiles and associate them with TAD boundaries. Our analyses reveal that CTCF binding is mainta

SUBMITTER: Kentepozidou E 

PROVIDER: S-EPMC6945661 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature