Characterizing the ribosomal tandem repeat and its utility as a DNA barcode in lichen-forming fungi.

ABSTRACT:

Background

Regions within the nuclear ribosomal operon are a major tool for inferring evolutionary relationships and investigating diversity in fungi. In spite of the prevalent use of ribosomal markers in fungal research, central features of nuclear ribosomal DNA (nrDNA) evolution are poorly characterized for fungi in general, including lichenized fungi. The internal transcribed spacer (ITS) region of the nrDNA has been adopted as the primary DNA barcode identification marker for fungi. However, little is known about intragenomic variation in the nrDNA in symbiotic fungi. In order to better understand evolution of nrDNA and the utility of the ITS region for barcode identification of lichen-forming fungal species, we generated nearly complete nuclear ribosomal operon sequences from

SUBMITTER: Bradshaw M 

PROVIDER: S-EPMC6945747 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature