Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing.

ABSTRACT: The human gut is colonized by trillions of bacteria that support physiologic functions such as food metabolism, energy harvesting, and regulation of the immune system. Perturbation of the healthy gut microbiome has been suggested to play a role in the development of inflammatory diseases, including multiple sclerosis (MS). Environmental and genetic factors can influence the composition of the microbiome; therefore, identification of microbial communities linked with a disease phenotype has become the first step towards defining the microbiome's role in health and disease. Use of 16S rRNA metagenomic sequencing for profiling bacterial community has helped in advancing microbiome research. Despite its wide use, there is no uniform protocol for 16S rRNA-based taxonomic profiling analysis. Ano

SUBMITTER: Shahi SK 

PROVIDER: S-EPMC6945761 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature