Metformin-repressed miR-381-YAP-snail axis activity disrupts NSCLC growth and metastasis.

ABSTRACT:

Background

Recent evidence indicates that metformin inhibits mammalian cancer growth and metastasis through the regulation of microRNAs. Metformin regulates miR-381 stability, which plays a vital role in tumor progression. Moreover, increased YAP expression and activity induce non-small cell lung cancer (NSCLC) tumor growth and metastasis. However, the molecular mechanism underpinning how metformin-induced upregulation of miR-381 directly targets YAP or its interactions with the epithelial-mesenchymal transition (EMT) marker protein Snail in NSCLC is still unknown.

Methods

Levels of RNA and protein were analyzed using qPCR, western blotting and immunofluorescence staining. Cellular proliferation was detected using a CCK8 assay. Cell migration and invasion were analyzed using

SUBMITTER: Jin D 

PROVIDER: S-EPMC6945774 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature