Acute ischemic stroke thrombi have an outer shell that impairs fibrinolysis.

ABSTRACT:

Objectives

Thrombi responsible for large vessel occlusion (LVO) in the setting of acute ischemic stroke (AIS) are characterized by a low recanalization rate after IV thrombolysis. To test whether AIS thrombi have inherent common features that limit their susceptibility to thrombolysis, we analyzed the composition and ultrastructural organization of AIS thrombi causing LVO.

Methods

A total of 199 endovascular thrombectomy-retrieved thrombi were analyzed by immunohistology and scanning electron microscopy (SEM) and subjected to ex vivo thrombolysis assay. The relationship between thrombus organization and thrombolysis resistance was further investigated in vitro using thrombus produced by recalcification of citrated whole blood.

Results

SEM and immunohistology analyses

SUBMITTER: Di Meglio L 

PROVIDER: S-EPMC6946479 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature