High-throughput screening for in planta characterization of VOC biosynthetic genes by PTR-ToF-MS.

ABSTRACT: Functional characterization of plant volatile organic compound (VOC) biosynthetic genes and elucidation of the biological function of their products often involve the screening of large numbers of plants from either independent transformation events or mapping populations. The low time resolution of standard gas chromatographic methods, however, represents a major bottleneck for in planta genetic characterization of VOC biosynthetic genes. Here we present a fast and highly-sensitive method for the high-throughput characterization of VOC emission levels/patterns by coupling a Proton Transfer Reaction Time-of-Flight Mass Spectrometer to an autosampler for automation of sample measurement. With this system more than 700 samples per day can be screened, detecting for each sample hundreds of sp

SUBMITTER: Li M 

PROVIDER: S-EPMC6946754 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature