Production of foot-and-mouth disease virus SAT2 VP1 protein.

ABSTRACT: The seven serotypes of foot-and-mouth disease virus (FMDV) differ on the surface exposed regions on the VP1, 2 and 3 proteins. Amongst the three, the VP1 protein has been produced the most for use in serotyping assays for some of the Euro-Asian serotypes. In this study the VP1 protein of the FMDV SAT2/ZIM/7/83 was expressed in Escherichia coli BL21 cells in Luria broth and EnPresso^® B media in shake flasks. Production was further developed and the VP1 protein was produced at 2.15 g L^-1 in fed-batch fermentations at 2 L scale. The protein formed insoluble inclusion bodies that were isolated, denatured and refolded. When tested in ELISA, the protein was found to be highly reactive with serum from a SAT2 vaccinated guinea pig, and not reactive to SAT1 and SAT3 antisera.

SUBMITTER: Mamabolo MV 

PROVIDER: S-EPMC6946773 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature