Establishment of NOAEL for intracavernous injections of human bone marrow-derived mesenchymal stem cells in rats.

ABSTRACT:

Purpose

To assess the possible negative health effects of human bone marrow-derived mesenchymal stem cells (hBMSCs) on fertility and early embryonic development following intracavernous injections in rats.

Materials and methods

A total of 88 Crl:CD(SD) male and female rats were equally divided into 4 groups in a random manner: control group (normal saline), low-dose group (2×10⁵ hBMSCs), moderate-dose group (1×10⁶ hBMSCs), and high-dose group (2×10⁶ hBMSCs). hBMSCs or normal saline was injected into the penis of the rats 3 times at 2-week-intervals prior to mating. We compared each group with respect to parameters of reproduction and histopathology.

Results

For male rats, various degrees of flushing and swelling were observed at the pe

SUBMITTER: Kim JK 

PROVIDER: S-EPMC6946817 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature