Genome-wide DNA methylation pattern in a mouse model reveals two novel genes associated with Staphylococcus aureus mastitis.

ABSTRACT:

Objective

Staphylococcus aureus (S. aureus) is one of the major microorganisms responsible for subclinical mastitis in dairy cattle. The present study was designed with the aim to explore the DNA methylation patterns using the Fluorescence-labeled methylation-sensitive amplified polymorphism (F-MSAP) techniques in a S. aureus-infected mouse model.

Methods

A total of 12 out-bred ICR female mice ranging from 12 -13 weeks-old were selected to construct a mastitis model. F-MSAP analysis was carried out to detect fluctuations of DNA methylation between control group and S. aureus mastitis group.

Results

Visible changes were observed in white cell counts in milk, percentage of granulocytes (GRN %), percentage of lymphocytes (LYM %), CD4+/ CD8+ ratio (CD4+/ CD8+), and histop

SUBMITTER: Wang D 

PROVIDER: S-EPMC6946959 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature